Fig. 1

miR-101-3p expression was upregulated in the calcified human aortic valves. A, B Photomicrographs of freshly isolated normal and calcified human aortic valves. C, D Alizarin red staining of normal and calcified human aortic valves, red staining indicating calcification (indicated by arrows). E, F Masson’s Trichrome staining of collagen deposition (blue staining indicated by arrows) of normal and calcified human aortic valves. G Small RNA deep sequencing results of miR-101-3p of normal and calcified human aortic valves. Data = means ± SEM. ***P < 0.001 vs Normal, n = 3. H qPCR analysis of miR-101-3p of normal and calcified human aortic valves. Scale bar = 200 μm. *P < 0.05 vs Normal, n = 3