Skip to main content
Fig. 3 | Molecular Medicine

Fig. 3

From: Inhibition of miR-101-3p prevents human aortic valve interstitial cell calcification through regulation of CDH11/SOX9 expression

Fig. 3

miR-101-3p regulated CDH11 and Sox9 protein expression and osteogenesis markers BMP2/Runx2 in HAVICs. A Western Blots against antibodies of Cadherin-11 (CDH11) (ab147215), Sox9 (AB5535, Millipore), BMP2 (ThermoFisher, 710022), and Runx2 (Santa Cruz sc-12488) in HAVICs at 72 h after transfection with negative control and various doses of miR-101-3p mimic. B–E Quantification of CDH11, Sox9, BMP2, and Runx2 protein expression normalized with β-actin. F Western Blots against antibodies of Cadherin-11 (CDH11) and Sox9 in HAVICs at 72 h after transfection with negative control and various doses of mir-101-3p inhibitor. G, H Quantification of CDH 11 and Sox9 protein expression normalized with β-actin. Data = means ± SEM. *p < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 vs Control. n = 3 repeats

Back to article page

Molecular Medicine

ISSN: 1528-3658