Fig. 5

Mimic of miR-101 downregulated CDH-11 and SOX9 expression and promoted osteogenesis in HAVICs treated by CM after transfection. A Western blot against antibodies of CDH11, SOX9, Runx2, and OPN in HAVICs transfected with miR-101-3p mimic for 48 h and then treated with CM for additional 48 h. β-actin serves as internal loading control. B–E Quantification of CDH11, SOX9, Runx2, and OPN normalized with β-actin. Data = means ± SEM. *p < 0.05, **P < 0.01 and ****P < 0.0001 vs Control; ⁺⁺⁺P < 0.001 vs Control, CM; ^P < 0.05 and ^^^P < 0.001 vs Mimic. n = 3 repeats