Fig. 6

Inhibition of miR-101 rescued downregulation of CDH-11 and SOX9 expression and prevented osteogenesis in CM-treated HAVICs. A Western blot against antibodies of CDH11, SOX9, Runx2, and OPN in HAVICs transfected with miR-101-3p inhibitor for 48 h and then treated with CM for additional 48 h. β-actin serves as internal loading control. B–E Quantification of CDH11, Sox9, Runx2 and OPN protein expression normalized with β-actin. Data = means ± SEM. **P < 0.01 and ***P < 0.001 vs Control; ⁺P < 0.05, ⁺⁺P < 0.001 and ⁺⁺⁺⁺P < 0.0001 vs Control, CM. n = 3 repeats