Fig. 7

miR-101 mediated ASPN expression in CM treated HAVICs through regulation of CDH11 and Sox9. A Western Blot against antibodies of ASPN in HAVICs transfected with miR-101-3p mimic or inhibitor for 48 h and then treated with CM for 48 h. β-Actin serves as internal loading control. B, C Quantifications of ASPN protein expression normalized with β-actin. *P < 0.05 and ****P < 0.0001 vs Control; ⁺P < 0.05 and ⁺⁺P < 0.01 vs Control, CM; ^^P < 0.01 vs Mimic. D Western Blot against CDH11, Sox9 and ASPN in HAVICs co-transfected with SiRNAs of CDH11 (10 nM) and Sox9 (1 nM) for 72 h. E–G Quantifications of WBs normalized by β-actin. Data = means ± SEM. ***P < 0.001 and ****P < 0.0001 vs Control. n = 3 repeats