Skip to main content
Fig. 2 | Molecular Medicine

Fig. 2

From: LncRNA GAS5 suppresses TGF-β1-induced transformation of pulmonary pericytes into myofibroblasts by recruiting KDM5B and promoting H3K4me2/3 demethylation of the PDGFRα/β promoter

Fig. 2

TGF-β1 induced pericyte–myofibroblast transformation in a dose- or time-dependent manner. Pericytes were exposed to different TGF-β1 concentrations (0, 1, 5, 10, and 15 ng/mL) for 12 h. A Western blotting analysis of the levels of pericyte markers (desmin, NG-2) and myofibroblast markers (α-SMA, collagen I). B RT-qPCR analysis of the expressions of GAS5 and PDGFR α/β in pericytes. C The expression of PDGFR α/β was evaluated via Western blotting. Pericytes were treated with 10 ng/mL of TGF-β1 at different time periods (1, 3, 6, 12, 24, and 48 h). D The expressions of GAS5 and PDGFR α/β were examined via RT-qPCR. E Western blotting analysis of the expression of PDGFR α/β. For A–E, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001

Back to article page

Molecular Medicine

ISSN: 1528-3658