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Fig. 5 | Molecular Medicine

Fig. 5

From: LncRNA GAS5 suppresses TGF-β1-induced transformation of pulmonary pericytes into myofibroblasts by recruiting KDM5B and promoting H3K4me2/3 demethylation of the PDGFRα/β promoter

Fig. 5

GAS5 recruited KDM5B to repress PDGFR α/β expression. A FISH assay was employed to observe the distribution of GAS5 in pericytes. B The nuclear and cytoplasmic expression of GAS5 in pericytes was detected via RT-qPCR. C Western blotting analysis of the protein levels of KDM5B and PDGFR α/β after KDM5B silencing. D/G The AnimalTFDB database predicted the binding sites of KDM5B in the promoter of PDGFR α/β. E/H ChIP assay was employed to examine the binding between KDM5B and the PDGFR α/β promoter. F/I Dual-luciferase assay confirmed the interaction between DKM5B and PDGFR α/β. J The interaction between GAS5 and KDM5B was assessed via RNA pull-down assay. K The direct binding between GAS5 and KDM5B was confirmed by RIP assay. L The binding between KDM5B and the PDGFR α/β promoter after GAS5 knockdown was detected via ChIP assay. M The interplay between GAS5 and KDM5B after GAS5 silencing or overexpression was examined via RNA pull-down assay. For A–M n = 3. *P < 0.05, **P < 0.01, ***P < 0.001

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Molecular Medicine

ISSN: 1528-3658