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Fig. 7 | Molecular Medicine

Fig. 7

From: LncRNA GAS5 suppresses TGF-β1-induced transformation of pulmonary pericytes into myofibroblasts by recruiting KDM5B and promoting H3K4me2/3 demethylation of the PDGFRα/β promoter

Fig. 7

GAS5 repressed TGF-β1-induced pericyte–myofibroblast transformation by regulating the KDM5B/PDGFR α/β signaling pathway. Pericytes were transfected with pcDNA3.0 containing KDM5B (KDM5B) and/or shGAS5 and then exposed to TGF-β1 (10 ng/mL) for 12 h. They were divided into the following groups: control, TGF-β1, TGF-β1 + NC, TGF-β1 + shGAS5, TGF-β1 + KDM5B, and TGF-β1 + shGAS5 + KDM5B. A, B Western blotting analysis of the expressions of KDM5B and PDGFR α/β after KDM5B overexpression. C Immunofluorescence staining to evaluate the expressions of desmin and α-SMA. D The protein levels of desmin, NG-2, α-SMA, and collagen I were assessed via Western blotting. For A–D, n = 3. *P < 0.05, **P < 0.01, ***P < 0.001

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Molecular Medicine

ISSN: 1528-3658