Fig. 5

The effect of OI on peritoneal macrophages from STZ-induced diabetic mice. The peritoneal macrophages from healthy and diabetic mice were stimulated with OI or NT for 24 h. A, C Representative western blots and protein quantification of Nrf2, HO-1, NQO-1, GCLC, GCLM, NLRP3, and iNOS in peritoneal macrophage lysates (n = 4 mice per group). The internal control was α-tubulin. Band intensity was measured by ImageJ. The band intensities were quantified and normalized to α-tubulin. B, D Representative western blots and activity quantification of the phosphorylated and total levels of ERK, p38, and JNK in peritoneal macrophage lysates. The internal control was α-tubulin. The results are shown as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001