Fig. 6
From: Caveolin-1 is involved in fatty infiltration and bone-tendon healing of rotator cuff tear
GATA6, downregulated in the supraspinatus tendon, inhibits adipogenic differentiation of TSCs by inhibiting Caveolin-1 expression. A The binding of GATA6 to the Caveolin-1 promoter region detected by ChIP assay. B The luciferase activity of Caveolin-1-WT and Caveolin-1-MUT in cells treated with oe-GATA6 determined by dual-luciferase reporter assay. C GATA6 and Caveolin-1 expression in TSCs treated with oe-GATA6 or combined with oe-Caveolin-1 detected by RT-qPCR. D Western blot of GATA6 and Caveolin-1 proteins in TSCs treated with oe-GATA6 or combined with oe-Caveolin-1. E ipid droplet formation of TSCs treated with oe-GATA6 or combined with oe-Caveolin-1 observed by oil red O staining. Cell experiments were repeated three times independently. *p < 0.05 compared with IgG, WT promoter, or oe-NC; # p < 0.05 compared with oe-GATA6 + oe-NC