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Fig. 7 | Molecular Medicine

Fig. 7

From: Mesenchymal stem cells reversibly de-differentiate myofibroblasts to fibroblast-like cells by inhibiting the TGF-β-SMAD2/3 pathway

Fig. 7

Reversible modulations on the TGF-β-SMAD2/3 signaling pathway in the uMSC-mediated MFB de-differentiation. A Network illustration of actin-binding, cadherin binding, SMAD binding, and associated DEGs. B Unsupervised clustering heatmap on genes participating in SMAD binding in FB, MFB, and co-cultured MFB samples. C GSEA analysis on regulated genes identified in MFB (MFB vs. FB). D GSEA analysis on regulated genes identified in co-cultured MFB (co-cultured MFB vs. MFB). E RT-qPCR assays on the expression of TGFB1, TGFBR1, SMAD2 and SMAD3 in cells from the 6 group settings: (1) Untreated FB; (2) MFB; (3) Short-term co-cultured MFB (FB-like cells); (4) Long-term co-cultured MFB; (5) FB-like cells treated with TGF-β1 without uMSC feeder layer; (6) TGF-β1-treated FB-like cells with additional uMSC feeder layer

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Molecular Medicine

ISSN: 1528-3658