Fig. 2
4-OI activates Nrf2 and alleviates inflammation and oxidative stress in LPS-induced AKI in vivo and in vitro. (A) Representative IHC staining images of Nrf2 (pointed to by the arrowhead) of renal tissues. Scale bar: 50 μm. (B) Representative western blot bands of Nrf2, HO-1, NQO1, and NLRP3 in renal cortex. (C) Relative band density of Nrf2, HO-1, NQO1 and NLRP3 in kidney cortex (n = 6). (D) Representative DHE staining images and IF staining images of F4/80. Scale bar: 50 μm. (E) Representative western blot bands of Nrf2, HO-1, NQO1, and IL-1β in BUMPT cells. (F) Relative band density of Nrf2, HO-1, NQO1, and IL-1β in BUMPT cells (n = 4). (G) qPCR analysis of mRNA of Il6 in BUMPT cells (n = 4). (H) Representative images of MitoSOX staining in BUMPT cells. Scale bar: 20 μm. (I) Semi-quantification analysis of MitoSOX fluorescence intensity. Each symbol (circle) represents an independent experiment. Data are presented as means ± SDs. *P < 0.05, **P < 0.01, ***P < 0.001; ns, not significant. IHC, Immunohistochemistry; IF, immunofluorescence; DHE, dihydroethidium; Nrf2, nuclear factor (erythroid-derived 2)-like 2; HO-1, heme oxygenase-1; NQO1, NAD(P)H quinone oxidoreductase 1