Fig. 4

4-OI attenuates LPS-induced mitochondrial ROS generation, and inflammation enhances mitophagy and subsequent apoptosis, which were partially abolished by the Nrf2 inhibitor. (A) Representative western blot bands of Nrf2, HO-1, NQO1, NLRP3, and cleaved caspase-3 in BUMPT cells. (B) Relative band intensity of Nrf2, HO-1, NQO1, NLRP3 and cleaved caspase-3 in BUMPT cells (n = 4). (C) Representative IF staining images of Nrf2 (red) in BUMPT cells. Scale bar: 20 μm. (D) Representative images of MitoSOX staining of BUMPT cells. Scale bar: 20 μm. (E) Representative western blot bands of Nrf2, HO-1, NQO1, IL-1β, and cleaved caspase-3 in murine kidneys. (F) Relative band intensity HO-1, NQO1, IL-1β and cleaved caspase-3 in murine kidneys (n = 6). (G) qPCR analysis of mRNA of Il6  in renal tissues (n = 6). (H) qPCR analysis of mRNA of Lcn2  in renal tissues (n = 6). (I) Representative western blot bands of LC3B-II, p62, TIMM23, and TOMM20 in BUMPT cells (n = 4). (J) Representative western blot bands of LC3B-II, p62, TIMM23, and TOMM20 in renal tissues. (K) Relative band intensity of LC3B-II, p62, TIMM23, and TOMM20 in BUMPT cells (n = 4). (L) Relative band intensity of LC3B-II, p62, TIMM23, and TOMM20 in renal tissues (n = 6). Each symbol (circle) represents an independent experiment. Data are presented as means ± SDs. *P < 0.05, **P < 0.01, ***P < 0.001; ns, not significant