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Fig. 4 | Molecular Medicine

Fig. 4

From: Receptor tyrosine kinase C-kit promotes a destructive phenotype of FLS in osteoarthritis via intracellular EMT signaling

Fig. 4

 C-kit drives FLS EMT signaling

(A) Validation of several shC-kit efficiency by Western blot (n = 3). (B-C) The expression of EMT makers (B) and Transwell invasive assay (C) in OA FLS with pCDNA-Ctrl, pCDNA-C-kit, shCtrl, or shC-kit transfection (n = 3, bar = 100 μm). (D) Morphological observation of DAPI (blue) and vimentin staining (green). Pseudopodia are marked by white arrowheads (bar = 20 μm). (E) The expression of inflammation-related markers (IL-6, IL-8, and MMP13) in FLSs (n = 3). (F) Illustration of co-culture of lenti-virus infected OA-FLS and chondrocytes. (G-H) After co-culture for 7 days, assessment of MMP13 (G) and Runx2 (H) of the chondrocytes (n = 5, bar = 100 μm)

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Molecular Medicine

ISSN: 1528-3658