Fig. 2

Overexpression of AURKA enhances the proliferation of HMEC-1 after high glucose and nutrient deprivation injury. Human microvascular endothelial cell (HMEC-1) cells were incubated with high glucose condition (HG) with nutrition deprivation (ND) for 12, 24, or 48 h (A). After that, the expression validation of Aurora kinase A (AURKA) (B) and cell viability were analyzed (C). AURKA was overexpressed via transduction with adenoviral vectors and treated with medium for 24 h (D), and the transduction efficiency was confirmed by the protein expression of AURKA (E). After 24 h of transfection, the cell proliferation was evaluated using bromodeoxyuridine (Brdu) labeling, and its quantification data were shown on the right. Pink spots were scored as positive-stained Brdu (scale bar, 50 μm) (F). The endothelial permeability of cells was tested using fluorescein isothiocyanate (FITC)-dextran (G). Flow cytometry analysis on cell cycle was analyzed (H), and the protein expression of cell cycle related proteins Cyclin B1 and CDK1 was measured using Western blotting (I). Data were represented as mean ± standard deviation (SD). ^*P < 0.05, ^**P < 0.01. Each dot indicated a biological replicate, n = 3