Fig. 3
From: The promotion action of AURKA on post-ischemic angiogenesis in diabetes-related limb ischemia
Overexpression of AURKA exerts a promoting effect on migration and tube formation ability of microvascular endothelial cells. The migration of cells was analyzed by scratch assay (scale bar, 200 μm) (A), and the percentage of the scratch migration rate was shown on the right panel (A). Red and green line indicated the boundary lines of scratch (A). Tube formation assay (B) was conducted to measure the tube formation ability of HMEC-1 cells with AURAK overexpression, and the tube-like structure length and bifurcation point numbers were quantified as shown on the right. Western blot assay were used to detect the expression of vascular endothelial growth factor A (VEGFA) in HMEC-1 cells (C). Protein levels of Vascular Endothelial Growth Factor Receptor 2 (VEGFR2), phosphatidylinositol 4,5-Bisphosphate 3-Kinase (PI3K), phosphorylated (p)-PI3K, serine-threonine protein kinase (AKT), and p-AKT were analyzed using Western blotting (D, E). Data were represented as mean ± SD. **P < 0.01. Each dot indicated a biological replicate, n = 3