Fig. 4
From: The promotion action of AURKA on post-ischemic angiogenesis in diabetes-related limb ischemia
AURKA overexpression protects cells against oxidative stress upon high glucose and nutrient deprivation stimulation. Detection of ROS production in HMEC-1 cells using the ROS-specific fluorescent probe 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) (A, left), and quantification of fluorescence intensities in indicated cells was displayed on the right (A). MFI, Mean fluorescence intensity. Relative levels of glutathione (GSH) (B) and malondialdehyde (MDA) (B) were examined in the cells. Detection of ROS production in HMEC-1 cells using the lipid ROS-specific fluorescent probe C11-BODIPY 581/591 and quantification of fluorescence intensities in indicated cells was displayed on the right (C). RT-qPCR (D) and Western blot (E) showed the levels of Solute Carrier Family 7 Member 11 (SLC7A11), Glutathione Peroxidase 4 (GPX4), Acyl-CoA Synthetase Long Chain Family Member 4 (ACSL4), and Arachidonate 5-Lipoxygenase (ALOX5) in indicated cells. Data were represented as mean ± SD. **P < 0.01. Each dot indicated a biological replicate, n = 3