Fig. 5
From: The promotion action of AURKA on post-ischemic angiogenesis in diabetes-related limb ischemia
AURKA overexpression enhances neovascularization in vivo. In vivo matrigel plug implant model using C57BL/6 mice was performed by subcutaneous injection of Matrigel mixed with AURKA overexpressing-adenovirus (AURKAOE) or its negative control (NCOE) (A). One week after, a gross matrigel plug image was taken (B). Slides of each matrigel plug were stained with hematoxylin and eosin (H&E) to observe angiogenesis response (asterisk marks) in vivo (C) (scale bar, 100 μm). Slides of each matrigel plug were subjected to CD31 immunofluorescence staining to visualize (left) and quantify (right) capillary density (D). Pink spots (asterisk marks) were scored as positive-stained CD31 (scale bar, 200 μm) (D). The hemoglobin concentration of matrigel plug was investigated, reflecting new blood vessels formation (E). The mRNA expression of AURKA was further validated in matrigel plug (F). Data were represented as mean ± SD. **P < 0.01. Each dot indicated a biological replicate, n = 8