Fig. 6
From: The promotion action of AURKA on post-ischemic angiogenesis in diabetes-related limb ischemia
AURKA overexpression promotes blood flow recovery in a mouse model of diabetic hind limb ischemia. To determine the role of AURKA in post-ischemic angiogenesis in vivo, ischemia injury and blood recovery were assessed in diabetic mice with critical limb ischemia (established by STZ injection and femoral artery ligation) (A). Blood perfusion (B) and the quantitative analysis (C) of hind-limb was measured at indicated time point using laser Doppler perfusion imaging (LDPI) after intramuscular injection of AURKA overexpressing-adenovirus (AURKAOE) or its negative control adenovirus(NCOE). Behavior function was assessed according to Tarlov score at indicated time-point. The time dependent expression changes of AURKA in gastrocnemius muscle was examined by RT-qPCR (E) and Western blotting (F). For C and E, data were represented as mean ± SD. For D, scatter plot represented the Tarlov score for individual mouse. **P < 0.01. Each dot indicated a biological replicate, n = 8