Fig. 7

AURKA overexpression protects muscle against diabetic ischemia injury. After 21 days of modeling, the AURKA mRNA (bar graph) and protein (bands) levels in gastrocnemius muscle tissues from all experimental groups were validated (A). Pathologic changes in gastrocnemius muscle tissues were evaluated using H&E staining (B, left), and the capillary density and myofiber cross-sectional area were determined (B, right). The black circle indicated capillaries. Scale bar, 100 μm (B). Immunohistochemistry (IHC) staining of Desmin on gastrocnemius muscle tissues to visualize muscle regeneration (C, upper), and the Desmin⁺ area was measures (C, lower). Scale bar, 50 μm. FITC-labeled Isolectin-B4 (50 μL) was injected via tail vein thirty minute before the mice were sacrificed. Identification of perfused blood vessels and capillaries in the gastrocnemius muscle was observed by dual immunofluorescence with Isolectin-B4 and CD31. Scale bar, 200 μm (D). Data were represented as mean ± SD. ^**P < 0.01. Each dot indicated a biological replicate, n = 8