Fig. 8

AURKA overexpression improved angiogenesis in ischemic gastrocnemius muscle of diabetic mice. After 21 days of modeling, dual-immunofluorescence analysis in gastrocnemius muscle tissues was conducted to visualize CD31 and α-smooth muscle actin (α-SMA). Scale bar, 200 μm (A). The capillaries (CD31⁺/α-SMA⁻, red box identification) (B) and arterioles (CD31⁺/α-SMA⁺, yellow box identifications) (C) was quantified. The VEGFA content of gastrocnemius muscle tissues was determined (D). Protein levels in gastrocnemius muscle tissues of VEGFR2 (E), p-PI3K (F), PI3K (F), p-AKT (G), and AKT (G) were analyzed using Western blotting (E–H). Data were represented as mean ± SD. ^**P < 0.01. Each dot indicated a biological replicate, n = 8