Fig. 9
From: The promotion action of AURKA on post-ischemic angiogenesis in diabetes-related limb ischemia
AURKA overexpression rescues ischemia-induced oxidative stress in murine gastrocnemius muscle. ROS production in the gastrocnemius muscle tissues was visualized by dihydroethidium (DHE) staining. Scale bar, 50 μm (A). 4-HNE expression in the gastrocnemius muscle tissues as shown by immunohistochemical (IHC). Scale bar, 50 μm (B). MDA and GSH content was measured using commercial assay kit (C). Besides, the mRNA (D) and protein (E, F) expression of GPX4 and SLC7A11 was detected. Data were represented as mean ± SD. **P < 0.01. Each dot indicated a biological replicate, n = 8