Fig. 1

Diabetic mice suffered from worse cerebral injury relative to the control mice after cerebral I/R. A Neurological scores were assessed in the control and diabetic mice underwent I/R or sham operation. The assessment was performed 36 h (blue bar) and 72 h (red bar) after operation. B Encephaledema volume of the brain slices were calculated according to the formula: V = ∑(A1 + A2)t/2. V: volume of encephaledema (mm³); A1 and A2: front and back encephaledema area of the slides (mm²); t: thickness of the slides (mm). C The brain tissues were stained by TTC solution. Pale area represented for encephaledema. The concentrations of TNF-α (D), IL-1β (E), and IL-18 (F) in the brain tissues were measured by ELISA kit. G The protein expression (GSDMD-N, GSDMD, caspase-1 p20, pro-caspase-1, ASC and NLRP3) in the control-sham, control-I/R, db/db-sham and db/db-I/R groups were detected by western blot. ^**P < 0.01, I/R, ischemia/reperfusion