Fig. 2
Knockdown of MALAT1 ameliorated cerebral injury and pyroptosis of the diabetic I/R mice. A The aberrant expressed lncRNAs in diabetic cerebral ischemia screened by bioinformatic analysis. B The expression of lncRNAs in mouse brain tissues. C Neurological scores were assessed in the diabetic mice underwent I/R or sham operation. Adenovirus harboring MALAT-1 short hairpin RNA (ad-sh-MALAT1) was infected into the diabetic I/R mice through intraventricular injection. The assessment was performed 72 h after operation. D The brain tissues were isolated from the treated mice and sliced into 2 mm thickness after they were euthanasia. Encephaledema volume of the slides were calculated according to the formula: V = ∑(A1 + A2)t/2. V: volume of encephaledema (mm3); A1 and A2: front and back encephaledema area of the slides (mm2); t: thickness of the slides (mm). E The brain tissues were stained by TTC solution. Pale area represented for encephaledema. F, G The protein expression of GSDMD-N, GSDMD, caspase-1 p20, pro-caspase-1, ASC and NLRP3 were separated by western blotting assay. The concentrations of TNF-α (H), IL-18 (I), and IL-1β (J) in the brain tissues were measured by ELISA kit. (n = 6), **P < 0.01, I/R, ischemia/reperfusion