Fig. 3

High glucose treatment aggravated cytotoxicity and pyroptosis of the H/R BV2 cells. A, B 72 h after the indicated treatment, BV2 cells were labelled with caspase-1 antibody and SYTOX, and counted by flow cytometry. C, D The cells were stained by PI and DAPI 72 h after the indicated treatment. PI positive rate was expressed as the proportion of PI positive cells (red) in the DAPI positive cells (blue). E LDH concentration of the BV2 cells were measured by ELISA kit 72 h after indicated treatment. The concentrations of IL-18 (F), and IL-1β (G) in the BV2 cells were measured by ELISA kit. H, I Western blot for GSDMD-N, GSDMD, caspase-1 p20, pro-caspase-1, ASC and NLRP3 of the BV2 cells 72 h after they received indicated treatment. The optical density (OD) of protein bands was indicated relative to corresponding loading control GAPDH. J MALAT1 levels were measured by RT-qPCR 72 h after the indicated treatment. (n = 6), ^**P < 0.01, OGD/R, oxygen–glucose deprivation/reoxygenation