Fig. 4

Downregulation of MALAT1 inhibited pyroptosis and cytotoxicity induced by high glucose and H/R of the BV2 cells. A Cspase-1/SYTOX positive cells were counted by flow cytometry. B LDH concentrations of the BV2 cells were detected with ELISA kit 72 h after indicated treatments. LDH amount was expressed as the fold to the LDH level of the NG group. IL-1β (C) and IL-18 (D) and concentrations in the BV2 cells were measured by ELISA kit 72 h after indicated treatments. E The protein expression of GSDMD-N, GSDMD, caspase-1 p20, pro-caspase-1, ASC and NLRP3 were separated by western blotting assay. (n = 6), ^**P < 0.01, H/R, hypoxia/reoxygenation; LDH, lactate dehydrogenase