Fig. 6

Knockdown of STAT1 reversed the effect of MALAT1 on pyroptosis induced by high glucose and H/R in BV2 cells. A The mRNA levels of STAT1 in the BV2 cells transfected with indicated ad-shRNA. B The cells were stained by SYTOX and cas-1. C LDH levels were measured by ELISA kit of the BV2 cells 72 h after indicated treatment. IL-1β (D) and IL-18 (E) concentrations of the BV2 cells were measured by ELISA kits 72 h after indicated treatments. F Western blot for GSDMD-N, GSDMD, caspase-1 p20, pro-caspase-1, ASC and NLRP3 of the BV2 cells 72 h after indicated treatment. (n = 6), ^**P < 0.01, H/R, hypoxia/reoxygenation; LDH, lactate dehydrogenase; NC, negative control