Fig. 7

Knockdown of STAT1 reversed the effect of MALAT1 on cerebral injury and pyroptosis of the diabetic I/R mice. A Neurological scores were assessed in vivo. B Encephaledema volume of the brain slices were calculated according to the formula: V = ∑(A1 + A2)t/2. V: volume of encephaledema (mm³); A1 and A2: front and back encephaledema area of the slides (mm²); t: thickness of the slides (mm). C The brain slices were stained by TTC solution. Pale area represented for encephaledema. The concentrations of TNF-α (D), IL-1β (E) and IL-18 (F) in the brain tissues were measured by ELISA kit. G, H The protein expression levels of GSDMD-N, GSDMD, caspase-1 p20, pro-caspase-1, ASC and NLRP3 in the mice underwent indicated treatment were detected by RT-qPCR 72 h after I/R modeling. (n = 6), ^**P < 0.01, I/R, ischemia/reperfusion