Fig. 2
From: RhoA promotes osteoclastogenesis and regulates bone remodeling through mTOR-NFATc1 signaling
The absence of RhoA impairs the production and function of osteoclasts. a, b BMMs from RhoAf/f and LCre; RhoAf/f mice cultured with M-CSF (40 ng/ml) and varied dosages of RANKL (a) or specified periods with 50 ng/ml RANKL (b). The osteoclast numbers were counted (right). n = 6, Scale bars, 200 µm. c Expression of osteoclast-specific genes in RhoAf/f and LCre; RhoAf/f mice osteoclasts, n = 6. d Representative Phalloidin staining images of BMMs cultured with M-CSF and RANKL for 4 or 7 days, and quantification of F-actin ring per well and nuclei per osteoclast. n = 6, Scale bars, 100 µm. e SEM (scanning electron microscope) analysis and quantification of bone resorption area of bone slides, n = 6, Scale bars, 20 µm. Mean ± s.d., *P < 0.05, **P < 0.01, Student’s t-test or ANOVA analysis