Fig. 3
From: RhoA promotes osteoclastogenesis and regulates bone remodeling through mTOR-NFATc1 signaling
RhoA-mTOR-NFATc1signaling enhanced osteoclastogenesis. a, b Western blot of NFATc1 in RAW264.7 cells infected with shRhoA (a) and in BMMs from RhoAf/f and LCre; RhoAf/f mice and treated with RANKL for 24 h (b). c Western blot of S6, p70- S6K, and Akt phosphorylation in RAW264.7 cells infected by shRhoA and treated with RANKL for 15 min. d Western blot of S6, p70-S6K, and Akt phosphorylation in BMMs from RhoAf/f and LCre; RhoAf/f mice. e Representative TRAP staining images from RhoAf/f and LCre; RhoAf/f mice treated with or without SC79 (10 µM) respectively. f TRAP staining of BMMs infected by lenti-RhoA and treated with or without rapamycin (10 nM). n = 6, Scale bars, 200 µm. Mean ± s.d., *,+P < 0.05, **P < 0.01, Student’s t-test or ANOVA analysis