Fig. 4

Rock enhanced the formation of osteoclasts by mTOR pathway. a Representative TRAP staining images of BMMs cultured with indicated doses of Y27632. b Expression of osteoclast-specific genes in BMMs cultured with 20 µM Y27632. c Western blot of NFATc1 in BMMs treated with Y27632 and RANKL. d Western blot of S6, p70-S6K, and Akt phosphorylation in BMMs treated with Y27632. e Co-IP of mTOR and Rock association in RAW264.7 cells with RANKL treated. f TRAP staining of BMMs infected with lenti-S6K1 and treated with or without Y27632. g Representative TRAP staining images of BMMs cultured with Y27632 and SC79 (10 µM). n = 6, Scale bars, 200 µm. Mean ± s.d., *^,+P < 0.05, **P < 0.01, Student’s t-test or ANOVA analysis