Fig. 5

In osteoclast precursors, RhoA gain-of-function reduced the bone mass and increases osteoclast activity. a Representative Micro-CT images of 3-month-old caRhoA and LCre; caRhoA mice tibias. b The parameters of tibias growth plate trabecular bone, n = 6. c Representative H&E and TRAP staining images of mice femurs. Scale bars, 200 µm (H&E) and 20 µm (TRAP). d The parameters of femur osteoclasts, n = 6. e Representative TRAP staining images of BMMs from caRhoA and LCre;caRhoA mice. Scale bars, 200 µm. f Representative Phalloidin staining images of BMMs, and the quantification of F-actin ring per well and nuclei per osteoclast, n = 3, Scale bars, 100 µm. g SEM analysis and quantification of bone resorption area of bone slides, n = 6, Scale bars, 20 µm. h Western blot of S6, p70-S6K and Akt phosphorylation in BMMs from caRhoA and LCre; caRhoA mice and treated with RANKL for 15 min. i Western blot of NFATc1 in BMMs from caRhoA and LCre; caRhoA mice and treated with RANKL for 24 h. Mean ± s.d., *P < 0.05, **P < 0.01, Student’s t-test