Fig. 2
From: FUT1-mediated terminal fucosylation acts as a new target to attenuate renal fibrosis
FUT1 accelerates EMT to promote renal fibrosis in HK-2 cells
HK-2 cells were transfected FUT1 plasmid with or without TGF-β1 (10 ng/mL) for 48 h. (A) The protein level of FUT1 in HK-2. (B) The mRNA level of FUT1 in HK-2. (C) The terminal fucosylation level (green) in HK-2 cells was analyzed by UEA-I staining. (D) The morphology of HK-2 transfected FUT1 plasmid with or without TGF-β1. (E) The protein level of fibrosis-related molecules in HK-2 was analyzed by western blotting. (F) The mRNA level of fibrosis-related molecules in HK-2 was analyzed by qPCR. (G) The protein level of EMT-related molecules in HK-2 was analyzed by western blotting. (H) The ability of migration was examined in treated HK-2 by cell migration assay. Scale bars = 100 μm. Values are expressed as the mean ± standard error of the mean from three independent experiments; ns, not significant, *P < 0.05, **P < 0.01