Fig. 5
From: FUT1-mediated terminal fucosylation acts as a new target to attenuate renal fibrosis
2-D-gal inhibits EMT via TGF-β/Smad pathway to ameliorate renal fibrosis
HK-2 cells were pre-treated with LY2109761 for 2 h ahead of treatment with 2-D-gal and TGF-β1. (A) The morphology of HK-2. (B) The protein level of fibrosis-related molecules in HK-2 was analyzed by western blotting. (C) The mRNA level of fibrosis-related molecules in HK-2 was analyzed by qPCR. (D) The protein level of EMT-related molecules in HK-2 was analyzed by western blotting. (E) The ability of migration was examined in treated HK-2 by cell migration assay. Values are expressed as the mean ± standard error of the mean from three independent experiments; ns, not significant, *P < 0.05, **P < 0.01