Fig. 6
From: FUT1-mediated terminal fucosylation acts as a new target to attenuate renal fibrosis
2-D-gal ameliorates renal fibrosis in UUO-induced renal fibrosis mice
C57BL/6 mice (n = 5) received UUO to establish renal fibrosis with or without 2-D-gal application via intraperitoneal injection (250 mg/kg) every other day. (A) Representative photomicrographs of H&E and Masson’s Trichrome Stain in kidney tissues of mice. (B) Representative photomicrographs of UEA-I (green) and TGF-βR I (red) expression in mice kidney by immunofluorescence analysis. (C) Representative photomicrographs of UEA-I (green) and TGF-βR II (red) expression in mice kidney by immunofluorescence analysis. (D) The protein level of fibrosis-related molecules in kidney tissues of mice was analyzed by western blotting. (E) The mRNA level of fibrosis-related molecules in HK-2 was analyzed by qPCR. (F) The protein level of p-Smad2, p-Smad3 and EMT-related molecules in kidney tissues of mice was analyzed by western blotting. Scale bars = 200 μm. Values are expressed as the mean ± standard error; ns, not significant, *P < 0.05, **P < 0.01