Fig. 1
From: miR-34a-5p as molecular hub of pathomechanisms in Huntington’s disease
Workflow for comprehensive identification of miR-34a-5p target networks in Huntington’s disease. Reynolds et al. described a decreased miR-34a-5p expression in R6/2 HD mouse model (Reynolds et al. 2018). An in-silico target prediction by miRwalk2.0 identified 12 801 potential miR-34a-5p target genes. An overrepresentation analysis of these potential target genes assigned 147 potential target genes to the KEGG pathway “Huntington’s Disease”. Out of these target genes we excluded already validated target genes of miRTarBase 8.0 and target genes without canonical miR-34a-5p binding sites. Out of the remaining 36 target genes, 14 target genes were already tested in Kern et al. (Kern et al. 2021) and 22 target genes were tested in this study using our automated dual luciferase reporter assay