Fig. 4

Western Blot analysis of hsa-miR-34a-5p over-expressing SH-SY5Y cells. SH-SY5Y cells were transfected either with ANC or miRNA Mimic. 48 h later, protein expression of HIP1 and NDUFA9 regulated by hsa-miR-7-5p, was detected with specific antibodies in a Western Blot analysis. The experiments were conducted in 3 independent experiments. A Western Blot Analysis of HIP1 protein expression regulated by hsa-miR-34a-5p over-expression. B Western Blot Analysis of NDUFA9 protein expression regulated by hsa-miR-34a-5p over-expression. C Quantitative Analysis of HIP1 protein expression regulated by hsa-miR-34a-5p over-expression. One asterisk represents a significant reduction of the luciferase activity with a p-value ≤ 0.05. D Quantitative Analysis of NDUFA9 protein expression regulated by hsa-miR-34a-5p over-expression. One asterisk represents a significant reduction of the luciferase activity with a p-value ≤ 0.05. E Protein–protein interaction networks of the 19 direct miR-34a-5p targets in Huntington’s disease using the STRING database version 11.5 (https://string-db.org/). The respective miR-34a-5p targets genes which are associated with the depicted pathways are colored as indicated in the diagram