Fig. 7

Glycyrrhizin treatment induces autophagy in H. pylori-infected mice and ameliorates gastric tissue damage (a–c) C57BL/6 mice (n = 5 per group) were treated with antibiotics every 7 days. Then after 7 days incubation period, mice were infected with the H. pylori SS1 strain thrice a week on alternate days. Mice were incubated for 14 days and then administered with or without glycyrrhizin GLZ (10 mg /kg body weight), every day for 4 weeks. After treatment mice were sacrificed, and gastric tissues and serum were collected. a Immunoblot showing the expression of HMGB1 and autophagy proteins (HMGB1, P62, LAMP1) of mouse gastric tissues. α-tubulin was used as a protein loading control. Densitometry analyses are represented graphically. b The expression of IL-6 was determined by ELISA in a microplate reader. c Histology images of Control (CON), H. pylori (HP) infected, and H. pylori-infected plus glycyrrhizin treated (HP + GLZ) gastric tissues at 20X and 40X respectively representing the inflammatory changes. Black arrows (↑) indicate gastric tissue damages. Graphs were represented as mean ± SEM (n = 3); Significance was determined by one-way ANOVA; *p < 0.05, **p < 0.01, ***p < 0.001