Fig. 4

C3G mitigated ferroptosis in the kidneys from I/R-AKI mice. A–C The content of iron, MDA and GSH were determined in kidney tissues of different groups. (n = 6); D–E RT-PCR results of GPX4 and ACSL4 of sham + C3G, sham, I/R and I/R + C3G group. (n = 6); F Western blot results of GPX4 and ACSL4 in kidney tissues. (n = 3); G Representative immunohistochemistry images of GPX4, ACSL4 and 4-HNE were captured respectively and analyzed. Scale bars = 20 μm. (n = 3). *p < 0.05, **p < 0.001. C3G cyanidin-3-glucoside, I/R-AKI ischemia/reperfusion-induced acute kidney injury, MDA malondialdehyde, RT-PCR reverse transcription polymerase chain reaction, GPX4 glutathione peroxidase 4, ACSL4 acyl-CoA synthetase long chain family member 4, 4-HNE 4-hydroxynonenal