Fig. 2

METTL3 expression varies after inducing T-cell activation and differentiation in vitro. a Left: the protein levels of the m⁶A-modifying enzymes METTL3, METTL14, ALKBH5, and FTO in naïve CD4⁺ T cells activated by anti-CD3 and anti-CD28 antibodies relative to control cells were determined by Western blot analysis. GAPDH was used as a loading control; right: quantification of protein levels, n = 4. b Total m⁶A modification level in naïve CD4⁺ T cells before and after activation was examined by m⁶A colorimetric quantification, n = 4. c Left: Western blot analysis of METTL3 protein expression among polarized Th1, Th2, Th17, Tfh, and Treg cell subsets relative to Th0, and β-actin was used as a loading control; right: quantification of METTL3 protein expression among different T-cell subsets, n = 5. (*p < 0.05, **p < 0.01, ns, no significance, two-way ANOVA with Sidak’s multiple comparisons test for a, unpaired two-tailed Student’s t test for b, one-way ANOVA with Dunnett’s multiple comparisons test for c)