Fig. 4
Pharmacological inhibition of METTL3 aggravates the lupus-like phenotype in a cGVHD mouse model. a Schematic diagram of the cGVHD model and STM2457 administration. b, c Representative dot plots showing the proportions of naïve T cells, TCM cells, TEM cells (b), and Treg cells (c) in the spleen and dLNs of cGVHD mice. d Representative dot plots showing the proportions of splenic Th1 and Th17 cells in cGVHD mice. e Quantification plots of cells from b to d, n = 6. Data are representative of 2 independent experiments. f ELISA comparing the ANA, IgG, and dsDNA antibodies in the serum of cGVHD mice treated with either DMSO or STM2457 (mean ± SEM). g Representative H&E staining images of kidney sections and the grades of glomerular lesions of cGVHD mice treated with either DMSO or STM2457. Scale bar: 25 μm. h Representative histological images showing C3 and IgG deposition in the kidneys of cGVHD mice treated with either DMSO or STM2457. Scale bar: 50 μm. (*p < 0.05, **p < 0.01, ns, no significance, unpaired two-tailed Student’s t test)