Fig. 1
From: Impact of St. John’s wort extract Ze 117 on stress induced changes in the lipidome of PBMC
Principle of fluorescence anisotropy assay. The fluorescent probe DPH is excited by light which is polarized by a polarization filter in vertical position. When the probe is in a fluid surrounding, e.g. a membrane, it rotates and the emitted light is depolarized. When the membrane gets more viscous the rotation is attenuated and the emitted light is more polarized. The fluorescence intensity is measured by applying parallel and perpendicular polarization filters to the excitation light plane