Fig. 1
From: Cryopreserved placental biopsies maintain mitochondrial activity for high-resolution respirometry
Placental biopsies preparations and respirometry protocol SUIT-11. A Placenta biopsies’ preparations flow chart. Fresh preparation, cryopreservation, and snap freezing procedures are represented as shown in materials and methods. Created in BioRender.com. B Representative HRR trace of fresh placental biopsy. Blue line: oxygen concentration (µM); red line: oxygen flux [pmol/(s*mg)]. Vertical lines indicate the addition of the indicated chemicals (G + M + P: glutamate, malate, pyruvate; CytC: cytochrome c; Succ: succinate; UC: uncoupler; Rot: rotenone; TMPD + Asc: N,N,N0,N0-Tetramethyl-p-phenyl-enediamine dihydrochloride, ascorbate; AZD: sodium azide). Respiratory states are indicated (LEAK: leak state; OXPHOS CI: complex I-dependent oxidative phosphorylation capacity; CI + CII: complex I + II-dependent oxidative phosphorylation capacity; ETS: maximum electron transfer system capacity; CII: uncoupled complex II-dependent respiratory state; ROX: resting oxygen consumption; CIV: complex IV activity assay). C Representative HRR traces of cryopreserved (upper panel, oxygen flux in green) and snap frozen (lower panel, oxygen flux in fuchsia) placenta biopsies