Fig. 2
From: Cryopreserved placental biopsies maintain mitochondrial activity for high-resolution respirometry
OCRs and respirometric parameters of fresh, cryopreserved, and snap frozen placental biopsies. A Oxygen flux values (pmol/(s*mg tissue) of Fresh placentas (n = 10), Cryopreserved placentas (n = 5) and Snap Frozen Placentas (n = 5) in the indicated respiratory states. B Net OXPHOS (left panel) and ET (right panel) capacities calculated as P-L and E-L respectively. C FCRs of respiratory states as a proportion of ETS state. D State 3 respiratory states of Fresh, cryopreserved (Cryo), and snap frozen (Snap) placentas before and after cyt c addition revealing mitochondrial integrity. E Representative immunoblotting analysis of OXPHOS complexes and vinculin as loading control in fresh, cryopreserved, and snap frozen placental protein extracts. * versus fresh saponin placental specimens [**p < 0.01, ***p < 0.001, ****p < 0.0001]; + versus cryopreserved placental specimens [+ p < 0.05, + + p < 0.01, + + + + p < 0.0001]; § versus snap frozen placental specimens [§p < 0.05, §§p < 0.01, §§§§p < 0.0001]. Values are expressed as mean ± SEM