Fig. 3
From: Cryopreserved placental biopsies maintain mitochondrial activity for high-resolution respirometry
Mitochondrial membrane potential and apoptotic activation after placental biopsies cryopreservation and snap freezing. A Confocal fluorescence images of placental sections stained with actin (green), MitoTracker (red), and DAPI (blue). Mitochondrial membrane potential quantifications in fresh, cryopreserved, and snap frozen conditions are provided (right graph). Scale bar: 50 μm. *versus fresh saponin placental specimens [****p < 0.0001]; + versus cryopreserved placental specimens [+ + + + p < 0.0001]. Values are expressed as mean ± SEM. B Left panel images: confocal immunofluorescences of placental sections stained for cyt c (green), TIM23 (red), and DAPI (blue). Scale bar: 50 μm. Right panel images: magnifications of left panel’s inserts showing cyt c/TIM23 colocalization. C Representative immunoblotting analysis of cleaved caspase 3 (upper panel), cleaved caspase 9, Bax (lower panel), and vinculin as loading control in fresh, cryopreserved, and snap frozen placental protein extracts. D Confocal immunofluorescences of placental sections stained for actin (green), cleaved caspase 3 (red), and DAPI (blue). Scale bar: 50 μm