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Fig. 2 | Molecular Medicine

Fig. 2

From: Myoblast-derived exosomal Prrx2 attenuates osteoporosis via transcriptional regulation of lncRNA-MIR22HG to activate Hippo pathway

Fig. 2

Myoblast-derived exosomal Prrx-2 facilitates osteogenic differentiation of BMSCs. (A) Morphology of isolated mouse BMSCs. (B) Flow cytometry confirmed the positive expression of CD105, CD90, CD73, and negative expression of CD45 and CD34 in BMSCs. (C) The internalization of PKH26-labeled exosomes by BMSCs was observed under a fluorescence microscopy. The expression of Prrx-2 in exosomes derived from Prrx-2-silenced C2C12 myoblasts was assessed by qRT-PCR (D) and Western blotting (E). (F) The calcium deposition of BMSCs was measured using Alizarin Red staining in the proliferation medium (PM), osteoblastic medium (OM), OM + Exo-shNC, OM + Exo-shPrrx-2 groups. (G) The ALP activity of BMSCs in the PM, OM, OM + Exo-shNC, OM + Exo-shPrrx-2 groups was determined. (H-M) qRT-PCR and Western blot analysis of the mRNA and protein levels of OCN, OPN, RUNX2, and BMP2 in the PM, OM, OM + Exo-shNC, OM + Exo-shPrrx-2 groups. * P < 0.05, ** P < 0.01 and *** P < 0.001

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Molecular Medicine

ISSN: 1528-3658