Fig. 7

YAP1 is a downstream target gene of miR-128. (A) miR-128 and (B) YAP1 expression in BMSCs transfected with miR-128 mimics or miR-128 inhibitor was detected by qRT-PCR. (C) Starbase database was adopted to predict the binding sites of miR-128 to YAP1 3′UTR. (D) The interaction between miR-128 and YAP1 was analyzed by dual-luciferase reporter assay. (E&F) Western blotting analysis of YAP1 protein levels in BMSCs after transfection with miR-128 mimics or miR-128 inhibitor. (G) Immunofluorescence staining analysis of the subcellular distribution of YAP1 in BMSCs. * P < 0.05, ** P < 0.01 and *** P < 0.001