Fig. 8

MiR-128 restrains BMSC osteogenic differentiation via targeting YAP1. (A) The overexpression efficiency of YAP1 in BMSCs was validated by qRT-PCR and Western blotting. (B) Alizarin Red staining was performed to detect the matrix mineralization of BMSCs from OM + mimics NC, OM + miR-128 mimics, OM + miR-128 mimics + vector, OM + miR-128 mimics + YAP1 groups. (C) ALP activity of BMSCs from multiple groups. (D-H) The mRNA and protein levels of OCN, BMP2, OPN, and RUNX2 in BMSCs were assessed by qRT-PCR and Western blotting. * P < 0.05, ** P < 0.01 and *** P < 0.001