Fig. 5

ATF5 and UPRmt related molecule expression in HK-2 cells incubated with HG for 0–48 h. A Immunofluorescence analysis were performed to access ATF5 nuclear translocation in HK-2 cells. Scale bar: 2 μm. A1 Semiquantification of green fluorescence density of ATF5. B Western blot analysis of ATF5 nuclear protein expression in HK-2 cells. B1 Relative band density of ATF5. C Western blot analysis of ATF5, HSP60, LONP1, FN, C-CAS3 expression in HK-2 cells with 30 mM HG exposure for 0–48 h. C1–C5 Relative band density. All data are presented as means ± SD; *p < 0.05 vs LG; ^#p < 0.05 vs 36 h HG. n = 3