Fig. 6

Knockdown of ATF5 downregulates the HSP60/LONP1 expression, exacerbated injury in HK-2 cells after 6 h HG exposure. A The efficiency of ATF5 siRNA3-mediated knockdown was verified by Western blot analysis. A1 Relative band density of ATF5. B Western blot analysis of ATF5 nuclear protein in HK-2 cells. B1 Relative band density. C Western blot analysis of ATF5, HSP60, LONP1, FN, C-CAS3 cellular proteins after transfecting ATF5-siRNA in HK-2 cells treated with HG for 6 h. C1–C5 Relative band density. D MitoSOX dye was used to detect mitochondrial ROS. Scale bar: 30 μm. E Representative microscopy images of FITC-Annexin staining. Scale bar: 30 μm. D1–E1 Semiquantification of Relative fluorescence intensity, the integrated optical density of MitoSOX/DAPI and FITC-Annexin V/DAPI were calculated by IPP. All data are presented as means ± SD; *p < 0.05 vs LG; ^#p < 0.05 vs 6 h HG + Neg-siRNA. n = 3